Protein_Domain
Part:BBa_K758004:Design
Designed by: Takaaki Takeda Group: iGEM12_KIT-Kyoto (2012-09-17)
GAL4, this part coding protein binds and activates the UAS sequence
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 725
Illegal PstI site found at 1368 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 1368
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 237
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 725
Illegal PstI site found at 1368 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 725
Illegal PstI site found at 1368 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 156
Illegal BsaI.rc site found at 1832
Illegal SapI site found at 521
Design Notes
PCR pGaTB to amplify GAL4 sequence.
Source
GAL4 protein, no promoter