Protein_Domain

Part:BBa_K758004:Design

Designed by: Takaaki Takeda   Group: iGEM12_KIT-Kyoto   (2012-09-17)


GAL4, this part coding protein binds and activates the UAS sequence


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 725
    Illegal PstI site found at 1368
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1368
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 237
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 725
    Illegal PstI site found at 1368
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 725
    Illegal PstI site found at 1368
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 156
    Illegal BsaI.rc site found at 1832
    Illegal SapI site found at 521


Design Notes

PCR pGaTB to amplify GAL4 sequence.


Source

GAL4 protein, no promoter

References